Evaluation of mesenchymal cells and dapsone for the treatment of dermonecrotic wounds caused by Loxosceles laeta venom in rabbits

Abstract This study aimed to evaluate the efficacy of mesenchymal stem cells (MSC), alone or associated with dapsone (DAP), in treating dermonecrotic wounds caused by Loxosceles laeta venom. Twenty-five male rabbits were distributed into five groups. Negative control received ultrapure water (C-), whilst all other groups injected 20 μg L. After 4 hours, each group one following treatments: PBS (C+), DAP, MSC, and DAP+MSC. Animals evaluated daily photographic records made for analysis wound area. Twelve days after, animals euthanized skin samples removed histological analysis. We observed that DAP showed best percentage contraction at day 3. In treatments using MSCs, a negative value was isolated as well lower association MSC + when compared PBS, probably, increase initial infammation after application cells, due fact MSCs secrete broad spectrum bioactive molecules such cytokines growth factors favor regeneration. Histologically, it C+ extensive areas necrosis, ulcers, neutrophilic infiltrate, mineralization. Collagen deposition MSC+DAP treatment, however vascularization remained unchanged. is first report treatment cutaneous loxoscelism more studies are needed determine its use an alternative therapy lesions spider.